Antibody
Generation

Monoclonal Antibody Generation Services Using the ADLib® System

The ADLib® system, a proprietary monoclonal antibody generation technology developted by Chiome, supports the generation of antibodies essential for antibody therapeutics, in vitro diagnostics and life science research.

ADLib® Service Lineup

	ADLib^®mAb generation	ADLib^®Affinity Maturation （KI-AMP）
Type	Monoclonal antibody generation	Affinity maturation
Overview	Antigen-specific antibody generation service using ADLib^® libraries	Affinity improvement services for existing antibodies (e.g. hybridoma-derived antibodies).
Period	About 3 weeks to report ELISA screening results. About an additional 2 weeks until reporting of antibody sequence information for candidate clones.	About 8 weeks to construct a strain expressing desired-existing antibodies, involving all steps from vector construction to cell line QC, where the target antibody sequence is introduced into the ADLib® system. This is followed by Affinity maturation for around 1.5 months (including diversification, screening, sequencing and SPR analysis).
Antigen types	Soluble proteins Peptides Antibodies (anti-idiotypic antibodies) Low molecular weight compounds	Soluble proteins Peptides Antibodies (anti-idiotypic antibodies) Low molecular weight compounds
Advantages	Rapid results. Completely in vitro system, no use of animals. Selection and screening using full body IgG. Efficient selection using a cell sorter. Human antibodies can be obtained directly (no need for humanisation)	The method is based on inducing mutations in the antibody genes within DT40 cells (proprietary technology) and the isolation of candidate clones that show a higher affinity to the antigen. The original technology has been further improved to achieve a shorter timeframe and lower cost compared to our conventional method.

Click here on technical information on the ADLib^® system

Click here on Chiome's affinity maturation technology

Service Workflows (basic plan)

A securely structured, step-by-step workflow. The invoicing process also follows a stepwise approach.

ADLib^®mAb generation workflow

ADLib^®Affinity Maturation（KI-AMP）workflow

Comparison with Other Antibody Generation Platforms

The ADLib^® system is a method for selecting antigen-specific antibodies from a library of full-length antibodies displayed on the cell surface. Although phage display is widely recognized as the same platform, it differs in technical characteristics across various aspects (refer to the table below). In addition, the construction of libraries and the screening process often rely on the unique know-how and technology developed by individual laboratories.

Other well-established methods, such as hybridoma and B-cell cloning, exhibit numerous differences from the cell-surface display. For example, the immunisation process is necessary.

	ADLib^®mAb generation	Phage display	Hybridoma	B cell cloning
Animal immunisation	Not required	Not required (Implemented in some cases)	Required	Required
Non-immunogenic/ Toxic antigens	Acceptable (Toxic antigens depend on the concentration)	Acceptable	Not acceptable	Not acceptable
Processing time	Short ※1	Short	Long	Long (Shorter than hybridoma)
Sequence identification	At an early stage ※2	At a late stage	At a late stage	At an early stage
For functional assay	Rapid (Culture supernatant/purified antibody)	Slow (Recombinant antibodies required)	Rapid (Culture supernatant/purified antibody)	Slow (Recombinant antibodies required)
Converting to IgG	not required	Required	not required	not required
Humanisation	not required	Required (Not required in some cases)	Required (Not required in some cases)	Required (Not required in some cases)
Affinity maturation	Practicable ※3	Practicable ※4	Another technology required	Another technology required

※1 10 days after initiation, clones are assessed by ELISA.
※2 Acquired immediately after ELISA for a wide range of clones.
※3 Chiome's proprietary technology (click here for information of affinity maturation).
※4 Error-Prone PCR/Site-Saturation Mutagenesis etc.

Enquiry - Order - Delivery

① Enquiry	Please contact us using the provided contact form. https://www.chiome.co.jp/inquiries/?id=en
② First hearing	We will ask you about your requirements, information and applications for the antibodies you intend to generate, in order to determine wheter or not to provide our service.
③ Face-to-face meeting	We will meet you in person or online to discuss the work process. Flexible planning in line with your needs.
④ Test plan & quotation	Following the meeting we will provide you with a comprehensive test plan and a correspoinding quotation based on your requirements.
⑤ Antibody generation work	As soon as we receive the customer's PO, ｗe initiate the anitibody generation project, progressing through each step. During this process, we communicate closely with the customer, including interim reports.
⑥ Delivery	Supernatant, purified antibodies, sequence information, interim reports and final reports to ensure quality service.

Efficient Work Organisation and Experienced Staff

Comprised of PhD experts and skilled staff.

Almost all work is carried out within our state-of-the-art laboratories. Our cohesive team enables us to respond rapidly and flexibly to a wide range of needs..
Through close communication between our experts and clients, you can monitor the service's progress and manage your project effectively.